Procedure Listings
ACETAMINOPHEN
Methodology: Colorimetric
Perform/Available: Daily  
Synonyms: Tylenol, Datril, Excedrin, Liquiprin Tempra
CPT: 82003
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - Red, 5ml
Transport/Storage - Refrigerate 2-8 C
Remarks - Centrifuge & separate within 4 hours.
Unacceptable Conditions - Hemolysis
Stability - Room temperature 14 days, refrigerate 14 days, frozen 14 days.
Use: Analgesic, found in many over-the-counter pain medications.
 
ACETONE (Semiquantative)
Methodology: Visual/Chemical
Perform/Available: Daily  
Synonyms: Ketone bodies
CPT: 82009
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8 C
Remarks -

Centrifuge & refrigerate promptly.

Unacceptable Conditions - Hemolysis
Stability - Frozen 14 days.
Use:

To detect ketoacidosis resulting from diabetes mellitus, alcoholism, stress, starvation dietary imbalance, vomiting and other metabolic disorders.

 
ALBUMIN
Methodology: Colorimetric
Perform/Available: Daily  
Synonyms: N/A
CPT: 82040
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8 C
Remarks - Centrifuge within 4 hours.
Unacceptable Conditions - Hemolysis
Stability - Room temperature 7 days, refrigerate 14 days, frozen 14 days.
Use: Albumin is decreased in these conditions: nephrotic syndrome, starvation, myelomas, cirrhosis of liver, major infections, surgical and accidental trauma, exudative enteropathy, eclampsia, uremia, acute infectious hepatitis, severe congestive heart failure, mesenteric lymphatic diseases and idiopathic hypoalbuminemia.
 
ALCOHOL (Alcohol-Medical or Alcohol-State)
Methodology: Colorimetric
Synonyms: Ethanol Level, Blood Ethyl Alcohol, ETOH
CPT: 82055
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - Red, 5m1
Transport/Storage - Refrigerate 2-8 C
Remarks - Do not open tube.  USe soap and water to prep arm, do not use alcohol pads.  Centrifuge within 3 hours.  If not to be performed within 3 hours, separate and refrigerate.
Unacceptable Conditions - N/A
Stability - Room temperature 3 hours, refrigerate 7 days, frozen 14 days.
Use: Ethanol is the most common toxic substance encountered in medico-legal cases. It may be ordered by the doctor (medical) or a police officer (Alcohol-State). Toxic levels may result in a coma.
Comments: Special Instructions: Use soap and water to prep the arm.
 
ALKALINE PHOSPHATASE
Methodology: Enzymatic
Synonyms: Alk Phos; Alkaline Phosphatase, serum
CPT: 84075
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8 C
Remarks - Centrifuge within 4 hours.
Unacceptable Conditions - Hemolysis
Stability - Room temperature 4 days, refrigerate 4 days, frozen 14 days.
Use: Evaluate hepatobiliary disorders and skeletal disorders with increased osteoblastic or chondroblastic activity. For detecting cirrhosis, alcoholism, primary and metastatic neoplasia in liver and bone.
 
ALKALINE PHOSPHATASE ISOZYMES
Methodology: Heat inactivation/Enzymatic
Perform/Available: Daily.  
Synonyms: Alk Phos Isoenzymes, Fractioned Alkaline Phosphatase
CPT: 84080
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8 C
Remarks - Centrifuge
Unacceptable Conditions - Hemolysis
Stability - Room temperature 4 days, refrigerate 4 days, frozen 14 days.
Use: Used to evaluate hepatobiliary disease and bone disease associated with increased osteoblastic activity.
Comments: Test Includes: Total Alk Phos, Alk Phos Isoenzymes
 
ALKALINE PHOSPHATASE STAIN
Methodology: Special Stain
Perform/Available: Monday - Saturday, 1St shift  
Synonyms: Alk Phosphatase Stain, Leukocyte Alkaline Phosphatase Stain, LAP
CPT: 85540
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 4 ml green
Transport/Storage - keep tube in dark
Remarks - Make 5-8 smears within 3 hours and keep in dark.
Unacceptable Conditions - Clotted, hemolysis
Stability - Room temperature 3 hours.
Use: Used to differentiate chronic leukemia from myelocytic leukemoid reactions.
Comments: Special Instructions: Test is only performed Monday through Saturday on 1st shift. Blood smears must be made on blood less than 3 hours old.
 
ALPHA FETOPROTEIN (Tumor or Marker)
Methodology: MEIA
Perform/Available:

Monday - Friday 1 shift only, before 1:00 pm.

 

 
Synonyms: AFP - tumor Note: Maternal AFP's are sent out
CPT: 82105
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5 ml red/gray
Transport/Storage - Refrigerate 2-8C
Remarks -

This test is for tumor marker only.  AFP as part of a maternal tri-screen; see Tri-Screen, Maternal.

Unacceptable Conditions - Severe hemoloysis
Stability -

Sample must be frozen after 24 hours.

Room temperature: 8 hours; Refrigerate: 24 hours; Frozen: 14 days.

Use: Elevated levels occur in several malignant diseases most notably nonseminomatous testicular cancer and primary heptocellular carcinoma.
 
ALT
Methodology: Enzymatic
Perform/Available: Daily  
Synonyms: SGPT, GPT, Alanine Aminotransferase, Glutamic Pyruvate Transaminase
CPT: 84460
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8 C
Remarks - Centrifuge within 4 hours.
Unacceptable Conditions - Hemolysis
Stability - Room temperature 3 days, refrigerate 7 days.
Use: Liver function test, screening test for hepatitis, cirrhosis and other liver diseases. ALT is not normally elevated after myocardial infarctions.
 
AMMONIA (NH3)
Methodology: Colorimetric
Perform/Available: Daily  
Synonyms: NH3
CPT: 82140
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 4ml green, on ice
Transport/Storage - On ice, separate and freeze if greater than 3 hours.
Remarks -

Centrifuge and separate if test not performed within 15 minutes.

Unacceptable Conditions - Specimen not on ice.
Stability -

Refrigerate 3 hours

Frozen 4 days

Use: Liver function test. Increased levels found in liver disease and Reye’s syndrome.
Comments: Special Instructions: Transport tube on ice to the laboratory within 15 minutes.
 
AMYLASE
Methodology: Enzymatic
Perform/Available: Daily  
CPT: 82150
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 6.5 ml red/gray; 24 hour urine collection
Transport/Storage - Serum, refrigerate; urine, refrigerate.
Remarks - Serum, centrifuge within 4 hours, do not frreeze; urine, see Urine Collection Procedure.
Unacceptable Conditions - N/A
Stability - Serum & urine, room temperature 7 days, refrigerate 14 days.  Body fluid, refrigerate 14 days.
Use: Diagnosis of pancreatitis, diseases of the pancreas and of the salivary glands.
 
ANA (Antinuclear Antibody)
Methodology: Auto EIA/Indirect fluorescent antibody
Perform/Available: Test performed Monday, Wednesday & Fridays, 1st shift.  
Synonyms: Antinuclear Antibody
CPT: 86038
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5 mlRed/gray
Transport/Storage - Refrigerate 2-8 C
Remarks - Reflex to ANA titer if screening positive.
Unacceptable Conditions - Heparinized plasma, avoid collection in gel tubes.
Stability -

Room temperature 8 hours

Refrigerate 48 hours

Frozen 14 days

Use: Screening test for Systemic lupus erythematosus. Positive Screening will reflex to ANA titers.
 
ANTI-STREPTOLYSIN 0 TITER (ASO)
Methodology: Latex agglutination
Perform/Available: Monday - Friday 1st shift only  
CPT: 86060
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 7.5ml Red/gray
Transport/Storage - Refrigerate 2-8C
Remarks - n/a
Unacceptable Conditions - Hemolysis
Stability -

Room temperature

Refrigerate 48 hours

Frozen 14 days

Use: To aid in diagnosing rheumatic fever, acute glomerulonephritis and erythera nodosum.
 
ARTERIAL BLOOD GASES
Methodology: Ion Selective Electrobe
Perform/Available: Daily  
Synonyms: Arterial blood gases, ABG’s
CPT: 82805
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - 2ml arterial blood in heparinized syringe on ice.
Transport/Storage - On ice.
Remarks -

Done at Grant County, North and South Units only. Transport to lab within 15 minutes, record O2 level.

Unacceptable Conditions - Venous specimen
Stability - On ice 15 minutes.
Use: To monitor patients on oxygen therapy. Results depend on both lung perfusion and proper heart function.
Comments:

Test includes: pH, pCO2, pO2, HCO3, total CO2, O2 sat & base excess.  Note: Label syringe with the patients type and amount of oxygen ie. room air, percentage or liters of 02 and vent, mask, nasal candula, etc.

***REMOVE NEEDLE AND CAP SYRINGE BEFORE TRANSPORTING***

 
ARTHROPOD, MACROSCOPIC
Methodology: Visual/Microscope
Perform/Available: Daily 1st shift.  
Synonyms: LICE, TICKS
CPT: 87168
Specimen 
Requirement:
 
Inpatient - n/a
Outpatient - Arthropod in clean sealed container
Transport/Storage - Room temperature
Remarks - Use clean container with sela tight lid.
Unacceptable Conditions - n/a
Stability - Room temperature 24 hours.
Use: Macroscopic exam of arthropod in order to identify.